![]() ![]() To collect blood, the hind leg should be immobilised in the extended position by applying gentle downward pressure immediately above the knee joint. Where sedatives contain peripheral vasodilators, doses should be low to avoid prolonged bleeding from the puncture site. If sedation is required on welfare grounds care should be taken due to the vasodilation action of some sedatives. This should including habituating mice to to the sound of the electric shaver to minimise additional stress. In contrast, the trained mouse is calm with a relaxed tail and open eyes the mouse's colour is normal, and it is easy for the technician to find the blood vessel and fill up the capillary tube.Įvery opportunity should be taken to habituate mice for low stress procedures. The untrained mouse is agitated, flipping a stiff tail, struggling and trying to get away its ears are flipped back, and eyes half shut it is hard for the handler to find the blood vessel as the blood flow is "turned off". Saphenous bleeding of an untrained (top) and trained (bottom) mouse. The Impact of Common Recovery Blood Sampling Methods, in Mice (Mus Musculus), on Well-Being and Sample Quality: A Systematic Review. Meyer N et al. (2020). Impact of three commonly used blood sampling techniques on the welfare of laboratory mice: Taking the animal’s perspective.(2014). Physiological and pathological impact of blood sampling by retro-bulbar sinus puncture and facial vein phlebotomy in laboratory mice. Journal of Pharmacology and Pharmacotherapeutics 1(2): 87-93. Blood sample collection in small laboratory animals. Journal of Applied Toxicology 21(1): 15-23. Diehl KH et al. (2001). A good practice guide to the administration of substances and removal of blood, including routes and volumes.* Saphenous or tail vein sampling are recommended over these approaches. General anaesthesia required non recovery Do you require more than one blood sample from the same mouse? YES The two tables below are designed to assist in determining the amount of blood to sample from the animal, and depending on that volume, the most appropriate techniques to use. How to decide on the most appropriate blood sampling technique for mice? On average, mice have around 58.5 ml of blood per kg of bodyweight.Ī mouse weighing 25 g would therefore have a total blood volume (TBV) of approximately 58.5 ml/kg x 0.025 kg = 1.46 ml. Further advice is given in the general principles. As a general principle, sample volumes and frequency of sampling should be kept to a minimum. The volume of blood removed and the frequency of sampling should be based on the purpose of the scientific procedure and the total blood volume of the animal. ![]()
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